Analysis by Experts
Reliable Data
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General Raman experiments, Raman mapping, SERS, polarization experiment, and temperature-dependent measurements are all possible.
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Raman measurements can be performed on both small samples viewable under a microscope and larger objects that can't be placed on the stage.
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Proper consultation before analysis leads to more efficient experiments and more valuable data.
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All measurements and analyses are conducted by a Ph.D. specializing in Raman spectroscopy.
Basic Measurement
Using standard micro-Raman spectroscopy, various materials can be measured without any pretreatment.
We will provide the measurement data, microscope images, and analysis conditions.
The image shows the Raman spectrum of a genetically modified rice leaf, in which carotenoid compounds were detected.
Raman Mapping
Raman mapping is a measurement method that acquires spectra at each point on a two-dimensional plane at intervals of at least 200 nm, and reconstructs an image based on a specific peak.
The image on the left below shows an MCF7 cell observed under a 100x microscope, while the image on the right is the Raman mapping image.
Through Raman mapping measurements, it is possible to detect the distribution of substances that cannot be observed with the naked eye.
Plarized Raman
The open system of the Ramanlab is arranged to be suitable for polarization experiments.
Polarized Raman experiments provide information about the orientation and symmetry of molecules or crystals.
The image shows polarized Raman spectra measured by rotating the sample in a parallel polarization setup with a crystal formed along the z-axis on the substrate.
SERS
Surface-Enhanced Raman Spectroscopy (SERS) amplifies Raman signals by up to ~10¹¹ times using localized surface plasmon resonance on metallic nanostructures (e.g., gold or silver). SERS effectively suppresses fluorescence, making it ideal for detecting low-concentration or highly fluorescent substances. Our RamanLab provides SERS measurement services using silver or gold-based substrates. The spectrum shown is from a Rhodamine 6G solution measured on a silver substrate.
Temperature Dependent Experiment
By performing Raman spectroscopy while varying the temperature of a material, it is possible to observe changes in molecular vibrations through the Raman spectra.
Our RamanLab is equipped with instruments capable of conducting Raman measurements across a wide temperature range from -195°C to 600°C.
The figure shows an example of spectral changes in cellulose as a function of temperature, demonstrating how material properties can be monitored through thermal variation.
Data Analysis
In addition to providing raw Raman measurement data, our RamanLab also offers a range of Raman data analysis services upon request. These include baseline correction, peak assignment, normalization, peak fitting, integral intensity analysis, intensity ratio calculation, and spectral comparison.
The figure shows an example of Lorentzian fitting analysis performed on a specific Raman peak of a given material.
